rat cytokine antibody arrays  (R&D Systems)

Journal: International Journal of Medical Sciences

Article Title: Hyperbaric oxygen protects against periodontal bone loss by modulating inflammation and bone remodeling via RANKL/OPG expression in ligature-induced periodontitis

doi: 10.7150/ijms.122857

Figure Lengend Snippet: HBOT reduces gingival cytokine and chemokine expression in ligature-induced periodontitis. (A) Representative cytokine array blots showing expression profiles of 10 key inflammatory mediators (red boxes) in gingival tissues collected at day 14 and day 28 from Sham, PD+RECOV (natural recovery), PD (periodontitis only), PD+EHBOT (early HBOT), and PD+LHBOT (late HBOT) groups. The targeted proteins included: 1. CINC-1 (CXCL1), 2. CINC-2α/β (CXCL3), 3. sICAM-1, 4. IL-1α, 5. IL-1β, 6. IL-1 receptor antagonist (IL-1ra), 7. LIX (CXCL5), 8. L-selectin (CD62L), 9. Thymus chemokine (CCL25), and 10. TIMP-1. (B) Quantitative analysis of cytokine and chemokine expression at day 14. Periodontitis induced marked increases in several pro-inflammatory cytokines and chemokines. Early HBOT significantly suppressed most inflammatory mediators compared to the PD and PD+RECOV groups. (C) Quantitative analysis at day 28. PD-induced cytokine elevation persisted, while both early and late HBOT treatments effectively reduced IL-1α, IL-1β, IL-1ra, sICAM-1, CINC-1, CINC-2α/β, LIX, and thymus chemokine levels. Notably, early HBOT more effectively reduced thymus chemokine expression than natural recovery. Data are presented as mean ± SD. n = 6 per group. Panel 2A shows a cytokine dot-array membrane (R&D Systems ARY008) from a single, intact exposure; the published panel was border-cropped only to remove blank margins (global linear contrast, no compositing). See Supplementary for the full, uncropped membrane and original X-film/RAW.

Article Snippet: Protein extracts (200 μg per sample) were applied to Rat Cytokine Antibody Arrays (ARY008, R&D Systems, Inc., USA), which detect a panel of 34 inflammatory cytokines, chemokines, and adhesion molecules.

Techniques: Expressing, Membrane

Journal: Bioengineering & Translational Medicine

Article Title: Schwann cells acquire a repair phenotype after assembling into spheroids and show enhanced in vivo therapeutic potential for promoting peripheral nerve repair

doi: 10.1002/btm2.10635

Figure Lengend Snippet: Schwann cells (SCs) assembled as spheroids show enhanced paracrine potential. (a) Relative mRNA levels of neurotrophic factors in SCs in a single‐cell suspension or spheroid configuration. ( n = 4). (b) Representative results of cytokine antibody array analysis and (c) corresponding signal intensity normalized to the positive control. ( n = 3). Data are presented as the mean ± SD. All p values were calculated using two‐tailed Student's t test. * p < .05; ** p < .01; ns, not significant.

Article Snippet: To characterize the harvested CM, a rat cytokine antibody array (ab133992; Abcam) was utilized to measure 34 cytokines simultaneously via a chemiluminescence‐based method according to the manufacturer's instructions.

Techniques: Suspension, Ab Array, Positive Control, Two Tailed Test

Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Biostimulatory Micro-fragmented Nanofiber-Hydrogel Composite Improves Mesenchymal Stem Cell Delivery and Soft Tissue Remodelling

doi: 10.1002/smll.202202309

Figure Lengend Snippet: (A) Representative immunofluorescent staining images showing the infiltrated endothelial cells (stained with RECA-1 in red and alpha smooth muscle actin, αSMA. in green) inside the matrices at POD 14 and 28. Scale bar: 100 μm. (B) Quantitative data presenting the infiltrated endothelial cells density in the matrix over 28 days (n = 6). (C) Immunofluorescent staining images showing the adipogenesis inside the matrix at POD 14 and 28; perilipin-1 was stained in green, and adiponectin-1 stained with Acrp-30 in red. Scale bar: 200 μm. (D) Profiling of immunomodulation (left) and tissue remodelling (right) cytokine proteins production in mfNHC and mfNHC-MSC matrix at POD 14 and 28. Statistical significance was calculated by two-tailed Student t-test. N.S.P>0.05, **P<0.01. Data are presented as Mean ± Standard Error of the Mean.

Article Snippet: To characterize the inflammation- and regeneration-related cytokines and chemokines mediated by the injected mfNHC and/or MSCs, a rat cytokine antibody proteome profiler array (ARY030, R&D Systems) was used to analyse the tissue samples collected at POD 14 and 28.

Techniques: Staining, Two Tailed Test

Journal: Theranostics

Article Title: Reversal of bleomycin-induced rat pulmonary fibrosis by a xenograft of human umbilical mesenchymal stem cells from Wharton's jelly

doi: 10.7150/thno.33741

Figure Lengend Snippet: Transplantation of HUMSCs reduced the activation of fibroblasts and promoted the expression of TLR-4 in the left lungs of rats with PF. (A) Left lung sections were labeled with anti-α-SMA antibody to indicate activated fibroblasts in the left lungs of each group. (B) Western blot images represent the contents of α-SMA in the rats' left lungs. Quantitative results of α-SMA from western blotting are shown. The results indicated that activated fibroblasts significantly increased from Day 7 to 49. The activated fibroblasts significantly decreased after the transplantation of high doses of HUMSCs. (C) Rat Acta 2 mRNA were extracted from left lung suspensions and analyzed by quantitative RT-PCR, demonstrating that a significant decrease in acta2 expression in the BLM+HUMSCs (HD) group on Day 49. (D) Cell number in bronchoalveolar lavage fluid (BALF) of the BLM+HUMSCs (HD) group reduced significantly compared with that in the BLM group on Day 49. (E) The immunohistostaining of anti-TLR-4 showed that some TLR-4 positive cells were localized in connective tissue (arrow heads), however, some in the alveoli (arrows). (F) To further explore whether TLR-4 expression was localized in M2 macrophage, the left lung tissue sections from the BLM+HUMSCs (HD) group on Day 49 were subjected to double-staining with anti-TLR-4 (green) and anti-CD163 (red) antibodies. (G) The contents of TLR-4 in the rats' left lungs were detected through western blotting. The quantitative results indicated that TLR-4 significantly increased following the transplantation of HUMSCs. (H) Quantitative analysis of real-time RT-PCR of rat TLR-4 mRNA in the left lung tissue lysate demonstrated that rat TLR-4 mRNA was increased in the BLM+HUMSCs (HD) group (n = 3/group). (I) The result of RT-PCR of human TLR-4 revealed that human TLR-4 was only found in cell lysate of A549 cell line, but not in the lungs of all groups. (J) Left lung samples from the Normal, BLM, and BLM+HUMSCs (HD) groups on Day 49 were subjected to Human Cytokine Antibody Array analysis. The results suggested that the transplantation of HUMSCs increased human FGF-6 and IGF-1 concentrations in rats with pulmonary fibrosis. (K) Analyses were conducted using Rat Cytokine Antibody Array. The results indicated that the transplantation of HUMSCs stimulated rats with pulmonary fibrosis to produce higher amounts of β-NGF, fractalkine, and GM-CSF in their left lungs. ✱ vs the Normal group, p < 0.05. # vs the BLM group, p < 0.05. ♠ vs the BLM+HUMSCs (LD) group, p < 0.05. ⧫ vs the BLM+HUMSCs (HD) group, p < 0.05.

Article Snippet: In addition, a RayBio® Rat Cytokine Antibody Array C2 (RayBio® AAR-CYT-2-8) was used to detect cytokines produced or secreted by the rat lung tissues (n = 3/group).

Techniques: Transplantation Assay, Activation Assay, Expressing, Labeling, Western Blot, Quantitative RT-PCR, Double Staining, Reverse Transcription Polymerase Chain Reaction, Ab Array